3  Run the demo

In the R session opened during installation, run the command below to process immune_human_2x. It combines the two demo GEM wells and produces a Seurat/Signac object containing the multimodal results.

names selects the final result by its exact name using all_of(). tar_make() also builds the dependencies needed for that result, but does not build every plot in the gallery.

R

targets::tar_make(
  names = tidyselect::all_of("multimodal_Seurat_object.immune_human_2x")
)

Keep the R session open until the command finishes. Progress messages report targets being dispatched, completed, or skipped because they are already up to date. A previous demo run on an AMD EPYC 7543 system with 16 logical threads took under 25 minutes and wrote about 6 GB; your runtime may differ.

3.1 Confirm success

After the run, the following command should return character(0), meaning the requested endpoint and its dependencies are up to date:

R

targets::tar_outdated(
  names = tidyselect::all_of("multimodal_Seurat_object.immune_human_2x"),
  callr_function = NULL
)

If names are returned, those results still need building. If the run failed, follow Troubleshooting, fix the reported cause, and run the same tar_make() command again. Completed results can be reused.

Continue to Inspect the demo results to read the object and find the associated files.